Abstract: Aeromonas as well as a serious pathogen of human and aquatic economic animals.this article from a turtle farm in Qingdao unexplained deaths of a pathogen isolated lesion site HBJY01,identification of the bacterium.Combination of traditional identification methods using the 16S rRNA and gyrB HBJY01 cloning method to identify the bacteria species.The results show that traditional identification methods and identification of molecular biology methods were consistent.Experimental results show that artificial infection HBJY01 infect healthy turtle,the mortality rate of 100%,symptoms and unexplained death of roughly the same turtle.Whichever is the same pathogen isolated from the liver lesions.

Key words: separation, identification, molecular taxonomy