Abstract: In this paper, thirty-two microsatellite markers were used to analyze DNA differences among of Amur sturgeon (Acipenser schrenckii), Kaluga (Huso dauricus) and their hybrids [H. dauricus (♀)×A. schrenckii (♂)]，in which 28 were amplified in the three species, and one was merely amplified in Amur sturgeon, and three were not be applied well in Kaluga. Results showed that there were five markers which were effectively identified in the three populations, among which the amplification products of HLJSX350 was 227 bp in Amur sturgeon, 209 bp in Kaluga, and a mixed band of 209 bp and 227 bp in their hybrids. In addition, the specific loci from a part of Amur sturgeon and Kaluga were distinguished effectively in the amplification products of HLJSX226, HLJSX329, HLJSX332, and HLJSX351. Therefore, combining part of those loci can effectively be used to identify those three species of sturgeons. These co-dominant markers provide a powerful tool to identify the sturgeons and conserved species.