关键词: 仿刺参, 硒, 蛋氨酸硒, 生长

Abstract: The effects of feeding regimes on growth and immune indices in juvenile sea cucumber（Apostichopus japonicus） with body weight of 0.745±0.3g fed the diet containing selenomethionine（0.5 mg/kg） were studied at water temperature ranging from 9℃ to 13℃ from March 10,2010,to May 9,2010 in a lab.Total of 150 individuals were selected,randomly stocked into tanks of each 50 cm ×40 cm ×30 cm at a rate of 10 individuals per tank,and fed the diet containing seleniomethionine once daily（Group 1）,once every 5 days（Group 2）,once every 10 days（Group 3）,once every 15 day（Group 4） and once daily for the diet without selenium（Group 5,as control group） with triplication for 60 days.The results showed that the sea cucumber fed the diets containing selenium had significantly higher survival than that in the control group（P 0.05）,even though they had no significant differences in the specific growth compared with the animals in the control group（P 0.05）.There were very significantly higher activities of superoxide dismutase（SOD）（78.34 U/ml） and glutathione（GSH）（2.87×10-5mol/L） in the Group 2 than those in the control group（P 0.05）.No significant difference in the concentration of GSH was found between Group 2 and Group 3,and Group 4（P 0.05）.Compared with the other groups,there were higher acid and alkaline phosphatase activities（2.42 U/ml,and 5.76 U/ml） in Group 1,without significant difference（P 0.05）.It is recommended that the sea cucumber be fed the diet containing seleniumomethionine every day for promotion of survival and immunity.

Key words: Apostichopus japonicus Selenka, selenomethionine, growth, immunity