›› 2011, Vol. 24 ›› Issue (4): 12-15,3.

• 研究论文 • 上一篇    下一篇

野生及养殖茴鱼体内ACP、AKP和CAT活力的比较研究

赵吉伟,李小龙   

  1. 中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨,150070
  • 收稿日期:2011-04-15 出版日期:2011-04-15 发布日期:2011-04-15
  • 通讯作者: 赵吉伟

Comparison of ACP,AKP and CAT activities in different tissues between wild and farmed Thymallus

  • Received:2011-04-15 Online:2011-04-15 Published:2011-04-15

摘要: 茴鱼是我国珍稀濒危冷水性鱼类,本研究分别对黑龙江茴鱼的野生群体及养殖群体肝、肾、肌肉组织中免疫相关的碱性磷酸酶、酸性磷酸酶及过氧化氢酶活力进行了测定。实验结果表明:两群体三种组织中AKP活力均表现为:肾脏〉肝脏〉肌肉;ACP活力均表现为:肾脏〉肌肉〉肝脏;而CAT活力表现为:肝脏〉肾脏〉肌肉。同时,肾脏及肌肉组织中,ACP活力野生群体明显高于养殖群体相应组织,而在肝脏中,则表现为养殖群体较高;CAT活力与AKP活力变化趋势相同,均为:养殖群体肝脏及肾脏组织中,CAT活力明显高于野生群体相应组织,而在肌肉中,则表现为野生群体较高。了解和掌握养殖群体及野生群体茴鱼不同组织各种免疫相关酶活力的变化趋势,对完善并推广茴鱼全人工繁育、养殖具有重要的指导意义。

关键词: 茴鱼, 野生, 养殖, 碱性磷酸酶, 酸性磷酸酶, 过氧化氢酶

Abstract: Thymallus is a kind of cold water fishes which is rare and endangered now.In this study,the activities of ACP,AKP and CAT related to immunity response were measured in the livers,kidneys and muscles of the wild and farmed populations.The results showed that AKP activities exhibited a tendency among the three tissues in both two populations;with the highest level in kidneys while the lowest in muscles.ACP activities were highest in kidneys while lowest in livers,and CAT activities were highest in livers while lowest in muscles.Meanwhile,ACP activities detected in kidneys and muscles in wild populations were significantly higher than those in farmed population,with a contrast situation in livers.In addition,activities of CAT and AKP in different tissues changed with the same tendency,which was identified to be higher in livers and kidneys of farmed populations while in muscles of wild populations.This study aimed to understand the changeable tendency of the immunity related enzyme activities in wild and farmed Thymallus populations,and might offer the important guidance to the artificial reproduction and culture of Thymallus.

Key words: Thymallus, wild population, farmed population, Alkaline phosphatase, Acid phosphatase, Catalase

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