分光光度法测定两种蟹类精子密度的研究

›› 2012, Vol. 25 ›› Issue (1): 35-37,5.

分光光度法测定两种蟹类精子密度的研究

管卫兵,杨牧川,王成辉

上海海洋大学海洋科学学院,上海,201306%上海海洋大学水产与生命学院,上海,201306

收稿日期:2012-01-15 出版日期:2012-01-15 发布日期:2012-01-15
通讯作者: 管卫兵

Measurement of Sperm Concentration of Two Crab Species by Means of Spectrophotometric Method

Received:2012-01-15 Online:2012-01-15 Published:2012-01-15

摘要/Abstract

摘要： 蟹类精子计数对其冷冻保存及种群生殖能力的探究有重要意义。为了提高计数蟹类精子数量的速度,建立了分光光度法测定蟹类精子密度的方法。比较了两种蟹类在不同波长（380nm、520nm、760nm）下吸光度（A）与精子密度（C）的关系。结果表明,在520nm波段下三疣梭子蟹（Portunus trituberculatus）精子密度和吸光度之间也存在线性回归关系,其回归方程是：A=0.058×C（R2=0.9928）;中华绒螯蟹（Eriocheir sinensis）精子吸光度与精子密度呈线性回归关系,其回归方程是：A=0.061×C（R2=0.9892）。统计检验表明,使用该方法测定蟹类精子数目,是一种快速而准确的方法,可以加速蟹类精子计数速度。

关键词: 三疣梭子蟹, 中华绒螯蟹, 分光光度法, 精子密度

Abstract: It is significant of rapid measurement of crab sperm counts to study sperm cryopreservation and population reproduction.A spectrophotomeric method was used to determine the sperm concentration of swimming crab Portuns trituberculatus and Chinese mitten crab Eriocheir sinensis at wavelengths of 380,520,and 760 nm.It was found that the sensitive and reliable rapid measurement of crab sperm counts was carried out at 520 nm.A linear relationship between sperm concentration（C） and photometric absorbance（A） was observed for sperm concentration of swimming crab and Chinese mitten crab.The best regression equation for the standard curve at 520 nm was expressed as A= 0.058×C（R2=0.9928） for the swimming crab and A=0.061×C（R2=0.9892） for Chinese mitten crab.Statistical analysis showed that this spectrophotomeric method was fast and accurate for sperm count.

Key words: Portuns trituberculatus, Eriocheir sinensis, spectrophotomeric method, sperm concentration

中图分类号:

S966.16

管卫兵;杨牧川;王成辉. 分光光度法测定两种蟹类精子密度的研究[J]. , 2012, 25(1): 35-37,5.

[1]	李赫，宋文华，李文宽，富丽静，姚福向，胡宗云，张涛，张健. 三种常用农药对中华绒螯蟹幼蟹的急性毒性研究[J]. 水产学杂志, 2013, 26(6): 44-47.
[2]	李绍戊;杨洪波;王荻;卢彤岩. 用紫外分光光度法测定嗜水气单胞菌的浓度[J]. , 2013, 26(2): 40-42.
[3]	王吉桥;刘晶;姜静颖;张东升. 中华绒螯蟹卵膜和腹肢的形态及卵附着机制[J]. , 2006, 19(2): 32-36.
[4]	胡鹏飞;王茜;戴伟;王晓梅. 长江水系中华绒螯蟹线粒体DNA的遗传多样性研究[J]. , 2006, 19(1): 1-5.
[5]	吴萍;邱高峰;楼允东. 中华绒螯蟹促雄腺结构变化对精子发生的影响[J]. , 2002, 15(1): 88-92.