›› 2012, Vol. 25 ›› Issue (2): 1-6.

• 研究论文 •    下一篇

鲫微卫星标记与几个生长性状的相关性分析

贾志武,郑先虎,匡友谊,于彬彬,孙效文   

  1. 中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨150070 大连海洋大学水产与生命学院,辽宁大连116023%中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨150070 上海海洋大学水产与生命学院,上海201306%中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨,150070
  • 收稿日期:2012-02-15 出版日期:2012-02-15 发布日期:2012-02-15
  • 通讯作者: 贾志武

Correlation Analysis on Four Growth Traits in Crucian Carp Carassius auratus by SSR and EST-SSR Markers

  • Received:2012-02-15 Online:2012-02-15 Published:2012-02-15

摘要: 鲫(Carassius auratus)遗传多样性丰富,具多种不同倍性的亚种,是研究鱼类进化的独特材料。本研究利用79个SSR标记和22个EST-SSR标记检测了鲫自交F2代的181尾个体的基因型,对体重、体长、体高和体厚4个生长性状进行了单标记回归分析。Permutation检验(10000次)结果显示:有22个标记分别与体重、体长、体高和体厚具有显著相关性(P〈0.05),其中,HLJYJ15和HLJYJ244与4个性状均呈极显著相关(P〈0.01)。本研究筛选的101个微卫星标记丰富了鲫分子标记数量,可用于鲫连锁图谱的构建和QTL定位研究;获得了4个与鲫性状相关的标记及基因型,为鲫的分子标记辅助育种(MAS)和生长性状的遗传机制研究提供了工具。

关键词: 鲫, SSR, EST-SSR, 体重, 体长, 体高, 体厚

Abstract: Crucian carp Carassius auratus is rich in genetic diversity, and becomes a good candidate for research of molecular markers or genes associated with those growth traits. In the current study, 79 SSR and 22 EST-SSR markers were chosen to analysis the genom- ic of 181 progenies from a crucian carp F2 family. A GLM (General Linear Model) procedure was used to analyze the correlation be- tween markers and four growth traits including body weight, body length, body depth and body width. The significance testing by Per- mutation test( 10 000 replicates), 22 makers were shown to fit the correlation from four growth traits (P〈0.05), very significant corre- lation (P〈0.01) for HLJYJ15 and HLYJ244. Markers and gene types related to the four growth traits were obtained from this research, which gives effective basis to the study of crucian carp marker-assisted breeding and genetic mechanism of growth traits.

Key words: Carassius auratus, SSR, EST-SSR, body weight, standard length, body depth, body width

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