扁吻鱼微卫星的筛选及群体多样性分析

›› 2012, Vol. 25 ›› Issue (3): 20-25.

扁吻鱼微卫星的筛选及群体多样性分析

郝卓然,梁利群,常玉梅,唐然,李世国,任波

中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨150070 上海海洋大学水产与生命学院,上海201306%中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨,150070

收稿日期:2012-03-15 出版日期:2012-03-15 发布日期:2012-03-15
通讯作者: 郝卓然

Isolation and Characterization of Microsatellite from Genome of Aspiorhynchus laticeps

Received:2012-03-15 Online:2012-03-15 Published:2012-03-15

摘要/Abstract

摘要： 本文采用磁珠富集法筛选新疆扁吻鱼（Aspiorhynchus laticeps Day）的微卫星分子标记,获得13个位点,同时随机抽取1000对鲤引物检测扁吻鱼同源位点的多态性,获得具多态性位点18对。应用31对引物对扁吻鱼与塔里木裂腹鱼（Schizthorax biddulphi）进行遗传多样性分析,共检测到110个等位基因,每个位点的等位基因数为2~6个,扁吻鱼群体平均多态信息含量为0.5353,平均观测杂合度为0.5306,平均期望杂合度为0.6057。表明扁吻鱼群体多态性较高,遗传多样性处于中等偏上水平。群体间遗传距离为0.4763,遗传相似系数为0.6211,表明两个群体为同科不同属的群体。

关键词: 扁吻鱼, 鲤, 塔里木裂腹鱼, 微卫星分子标记, 遗传多样性

Abstract: In this study,magnetic beads enriched method was used to isolate microsatellite DNA from Aspiorhynchus laticeps Day genome and 13 sites were found.One thousand pairs of primers from common carp Cyprinus carpio randomly were chosen to test for the cross-species amplification in Aspiorhynchus laticeps Day,and 18 primers were amplified successfully.Polymorphism of all these 31 distinct loci was assessed using 24 Aspiorhynchus laticeps Day individuals and 19 Schizthorax biddulphi Gvnther,and a total of 110 alleles were detected.The Aspiorhynchus laticeps Day was shown to have average PIC of 0.5353,Ho of 0.5306,and He of 0.6057,indicating that the polymorphism of Aspiorhynchus laticeps groups is high and the genetic diversity is in the mid to upper level.The Aspiorhynchus laticeps Day and Schizthorax biddulphi Gvnther were found to show genetic distance of 0.6211 and genetic similarity coefficient of 0.4763,indicating that the two groups is of different genera in the same family.

Key words: Aspiorhynchus laticeps Day, Cyprinus carpio, Schizthorax biddulphi Gvnther, microsatellite marker, genetic diversity

中图分类号:

Q347

郝卓然;梁利群;常玉梅;唐然;李世国;任波. 扁吻鱼微卫星的筛选及群体多样性分析[J]. , 2012, 25(3): 20-25.

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