›› 2012, Vol. 25 ›› Issue (4): 16-18,2.

• 研究论文 • 上一篇    下一篇

锦鲤尾鳍细胞系(KF—H)的建立

薛淑群,孙中武,孙效文   

  1. 中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨,150070%东北林业大学生命科学学院,黑龙江哈尔滨,150040
  • 收稿日期:2012-04-15 出版日期:2012-04-15 发布日期:2012-04-15
  • 通讯作者: 薛淑群

Establishment of Koi's Caudal Fin Cell Line (KF-H)

  • Received:2012-04-15 Online:2012-04-15 Published:2012-04-15

摘要: 本研究建立了锦鲤(Cyprinus carpio)尾鳍细胞系。染色体数目、核型及DNA含量等实验,发现锦鲤体细胞和锦鲤培养细胞无显著性差异,染色体数目和DNA含量符合比例关系,建立的锦鲤尾鳍细胞系已形成了稳定的遗传性状,命名为KF—H。

关键词: 锦鲤尾鳍细胞系, 染色体, 核型分析, DNA含量

Abstract: The cell line of a caudal fin from koi (Cyprinus c arpio) was established in this study. The detection of the chromosome number, karyotype and DNA content demonstrates that there were no significant differences between the somatic cells and the culture cells; that the chromosome number and the DNA content were in accordance with the proportionate relationship. The established cell line of the koi' s caudal tail fin named as KF-H had the stable heredity.

Key words: Koi's caudal fin cell line, chromosome, karyotype analysis, DNA content

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