Abstract: The polymorphic primers for Dolly Varden Salvelinus malma as a rare and endangered cold-water species in China was screened from char S.confluentus by microsatellite,and the genetic diversity of the Yalu River population was analyzed.The results showed that 5 microsatellite primers screened from 10 microsatellite primers in Salvelinus malma were polymorphous in Dolly Varden.There were the number of alleles(Na) of 6~22(average 15),heteroerozygosity(H) of 0.4063~0.9333(average 0.7851) and polymorphic information content(PIC) of 0.716 4~0.919 8(average 0.8536) in Yalu River population of Dolly Varden.The findings indicated that the 5 primers from S.confluentus provided a good versatility in dolly varden,and a high level of genetic diversity in Yalu River population,which provides a genetics theoretical basis for the protection and management of germoplasm resources of Dolly Varden in China.

Key words: Salvelinus malma, microsatellite, genetic diversity, Yalu River