Abstract: Authors improved method of fish lymphocyte culture using whole blood short-term centrifugation to purify lymphocyte. The separation is good, and can keep the serum in the supernatant so that the method can stimulate growth and multiplication of the lymphocytes. Also, the chromosome preparation gives a good result, having lots of mitotic figure and clear background, which is very suitable for research fields ofkaryotype analysis, in situ hybridization, and so on.

Key words: lymphocyte culture, chromosome preparation, mitotic figure