The effects of probiotics I containing primarily Bacillus subtilis and probiotics II containing a mixture of various microorganisms on weight gain,food conversion ratio and drug cost of common carp（Cyprinus carpio）,Crucian carp（Carassius auratus） and grass carp（Ctenophyargodon idellus） in culture ponds in Heilongjiang and Liaoning provinces.The probiotics,alone or together,were showed to have influence on weight gain,the food conversion ratio and the drug cost in the fish culture.There were 423.57% higher weight gain in Heilongjiang province and 90% higher weight gain in Liaoning province in the ponds treated with the probiotics used together,than that in the control ponds with lower drug production cost of 29% in Heilongjiang province and 56.25% in Liaoning province.The food conversion ratio was found to be decreased by 0.35 and 0.05 in the ponds treated with probiotics used together,indicating that the probiotics have important influence on the profit of grass carp culture in Liaoning province and of common carp farming in Heilongjiang and have a promotion of green aquaculture with less drugs,less feed and more profits.

The optimum temperature for growth and feeding was studied in lenok Brachymystax lenok weighing 1.72±0.24g at of 6℃,11℃,16℃,and 21℃for 7 days in a laboratory.The results showed that the specific growth rate（SGR）,daily weight gain（DWG）,food conversion efficiency（FCE）and feed intake（FI） were found significantly higher than those in other groups at 16℃.The correlations between these parameters and water temperature（T） were described by nonlinear regression models： SGR=（-2.601）＋（0.887）T＋（-0.027）T2（P0.01,R= 0.979）,FI=（0.111）＋（0.292）T＋（-0.009）T2（P0.01,R=0.990）,FCE=（52.828） ＋（11.951） T＋（-0.393） T2（P0.01,R=0.933）.It is concluded that the optimum temperature was showed to vary from 15.20℃ to 16.43℃.

An experiment was carried out to study the effect of temperature of 12℃,15℃,18℃.20℃ and a running water control（range 4 to 7℃,average 6℃） on growth for 30 days（starting on the day fry floating in 12℃） in a full-sib all female rainbow trout（Oncorhynchus mykiss） family.Further,all the treatment groups were moved to the same condition as the control group in running water for another 90 days.On the day of 30,60,90,120 post fry floating,the growth traits,such as weight and length of the juvenile trout were measured for the treatment and control groups.Analyses of variance and Tukey-Kramer HSD test were used to determine if significant（P0.05） differences existed between the groups.The results of variance analysis showed that,for the body weight on the age of 30 day,the largest mean was 18℃ group,in turn,the 15℃,20℃,12℃ and the lowest was the control;for the body length,the largest mean was the 15℃ group,in turn,the 12℃,18℃,20℃ and the lowest was also the control.The results of Tukey-Kramer HSD test showed that the highest order for the body weight on the age of 30 day was 18℃ and 15℃ groups and the highest order for the body length on the age of 30 day was 15℃ group.On the age of 60 days,12 ℃,15 ℃,18 ℃,20 ℃,the body weight of all the four treatment groups were still significant higher than the control group（P 0.001）,the largest mean was still the 15℃ group,,this state remained on the age of 90 and 120 days.The above results showed that 15 ℃ was probably the optimal growth temperature of juvenile fish for the all female rainbow trout family.Just giving a 15 ℃ temperature control for 30 days,the all female juvenile fish could continuous remain significantly growth advantage during the next three months.

Thymallus is a kind of cold water fishes which is rare and endangered now.In this study,the activities of ACP,AKP and CAT related to immunity response were measured in the livers,kidneys and muscles of the wild and farmed populations.The results showed that AKP activities exhibited a tendency among the three tissues in both two populations;with the highest level in kidneys while the lowest in muscles.ACP activities were highest in kidneys while lowest in livers,and CAT activities were highest in livers while lowest in muscles.Meanwhile,ACP activities detected in kidneys and muscles in wild populations were significantly higher than those in farmed population,with a contrast situation in livers.In addition,activities of CAT and AKP in different tissues changed with the same tendency,which was identified to be higher in livers and kidneys of farmed populations while in muscles of wild populations.This study aimed to understand the changeable tendency of the immunity related enzyme activities in wild and farmed Thymallus populations,and might offer the important guidance to the artificial reproduction and culture of Thymallus.

Hybridization of large yellow croaker（Pseudosciaena crocea,♀） with mean body weight of 405 g,and mean body length of 27.9 cm.× spotted maigre（Nibea albiflora,♂）with with mean body weight of 390 g,and mean body length of 24.8 cm was artificially performed by dry fertilization and the embryonic development was observed in October of 2008.The pelagic fertilized eggs were 1.18mm in diameter,and had one oil globule with 379.8μm diameter in average.The fertilized eggs were incubated in sea water at a water temperature of 25.8 ~ 26.2℃ and a salinity of 26 and hatched by 18 h 55 min.The embryonic development of the hybrids was divided into egg cleavage stage,blastula stage,gastrula stage,heart beating stage,muscular contraction stage and hatching stage.The newly hatched larvae had total body length of 2.75 ~ 2.98 mm,with deformity of about 30%,fertilization success of 14.4%,and hatching rate of 31.9%.About 50000 larvae were obtained,beginning to feed rotifer as natural food three days after hatching,and ingesting copepods as food seven days after hatching.

The growth of 2-year-old commercial juvenile Acipenser schrenckii in pond-culture were studied.The results showed that the juveniles＇ mean total length which was 40.36cm and body weight which was 186.29g increased by 71.04% and 568.54% respectively after 60 days rearing.There was a power function relationship： W=0.0015L3.2208,R2=0.9662,between body weight and total length.The daily increasing weight,RIR,GC and GT of juveniles increased first,and reached the maxmium between 20 days and 40 days after the experiment starting,and then decreased.K ranged from 0.2834 to 0.3927.The SGR was unrelated with water temperature.The body weight and total length CV had no significantly change.

The norfloxacin（NFX） concentrations in plasma,liver and kidney were monitored 0.15,0.25,0.5,0.75,1,1.5,2,4,6,8,12,24,48,72 h after single intraperitoneal injection of 30 mg NFX /kg bodyweight in rainbow trout Oncorhynchus mykiss with body weight of 100 ± 10 g at a single dosage at water temperature of 15±2℃ by high performance liquid chromatography（HPLC） to study the pharmacokinetics of NFX.The NFX concentrations were shown to be described as a two-compartment open model with 1st order absorption in the plasma,liver and kidney.The T1/2α was found 0.866 h in the plasma,1.985 h in the liver and 0.388 h in the kidney,and the T1/2β was 31.369h in the plasma,36.402h in the liver and 30.975 h in the kidney.The AUC was described as 308.005 μg/mL·h in the plasma,622.721 μg/g·h in the liver,and 794.362 μg/g·h in the kidney.

The chemiluminescence immunoassay（CLIA） was used to detect the residues of Furazolidone,Chloramphenicol,Enrofloxacin and organic phosphorus in several edible shellfish（escallop Argopectens irradias,hard clam Meretrix meretrix,surf clam Mactra chinenesis,short necked clam Ruditapes philipinarum,burnt-end ark Anadara uropygimelana） in Dalian.The result showed that the drug residues were disproportionately detected in the test shellfish.But the extent of the drug residues was found within the standards of food hygiene,in line with food hygiene and safety standards.

Aeromonas as well as a serious pathogen of human and aquatic economic animals.this article from a turtle farm in Qingdao unexplained deaths of a pathogen isolated lesion site HBJY01,identification of the bacterium.Combination of traditional identification methods using the 16S rRNA and gyrB HBJY01 cloning method to identify the bacteria species.The results show that traditional identification methods and identification of molecular biology methods were consistent.Experimental results show that artificial infection HBJY01 infect healthy turtle,the mortality rate of 100%,symptoms and unexplained death of roughly the same turtle.Whichever is the same pathogen isolated from the liver lesions.

The different concentrations of Methylene Blue,Formaldehyde,Potassium Permanganate（KMnO4）,Sodium Chloride（NaCl）,and Malachite Green solution were used to control the impact of Saprolegniasis on eggs of Songpu mirror carp at water temperature of 24±1℃.The results showed that Methylene Blue solution（1100.00mg/L,10 min） and Formaldehyde solution（6000.00mg/L,10min） had a stronger inhibitory effect on Saprolegniasis and no significant influence on hatching rate.After the eggs was immersed in Methylene Blue solution for 10 min,the effective time lasted for 29.97 h,and abnormal rate of fries were found the lowest（only 1.55%）,while KMnO4 and NaCl solution did not show on significant inhibitory effects on the Saprolegniasis.In this experiment,we can draw a conclusion that,instead of Malachite Green solution,Methylene Blue solution can be used to prevent or control the Saprolegniasis.

Two Chinese herbal medicine compounds（AB,CD） were used as additives in order to estimate effects of the Chinese medicine on lysozyme activities in different tissues including hepatopancrease,serum,spleen and gills of common carp Cyprinus carpio L with body weight of g..The common carp were fed the diets containing two Chinese herbal medicine compounds at feeding rate of 2.0% and with the control group fed the basic diets at the same feeding rate for 28 days.Blood serum and the tissues samples were collected 1 day after stopping feeding while the lysozyme activity were assayed.The results showed that the two Chinese medicine compounds elevated the lysozyme activity in the common carp.The significant increase in lysozyme activity was found in the serum and tissue samples in CD groups compared with that in the control group.The significant increase in ysozyme activity in the fish in AB group except for the hepatopancrease according to that in the control group.There were no obvious differences between the two experimental groups except in the hepatopancrease.The two Chinese medicine compounds was found to enhance the common carp immunity with little difference.

The prerequisite of marker-assisted selection（MAS） is to find molecular markers or genes associated with those economic traits which are mostly controlled by a number of genes and inherited as quantitative traits.In this study,300 microsatellite markers were used to analyze the genotypes in the reference panel contained 92 individuals in a F2 family obtained by mating of F1 hybrids derived from crossing Barbless common carp（Cyprinus pellegrini） with Hebao？cold tolerance red common carp（Cyprinus carpio wuyuanensi）,and four economic traits including standard length,body width,body depth and body weight were tested using GLM（General Linear Model） single marker of the regression.The determination of the threshold values by 10000 Permutation test showed that 23 markers had significant correlation（P 0.05） with the four traits,in which HLJ44,HLJE28,HLJ1148,HLJ1329,HLJ464,and HLJE469 had very significant correlation with the standard length,body width,body depth and body weight（P 0.01）.In addition,the genotypes of these correlative loci captured were determined by Daucan＇s in SPSS13.0 software,and 75 segregation distorted microsatellites were detected whereas 11 segregation distorted microsatellites had correlation with the traits.Genotype deviate index describes the content of those genotypes deviation,the possibility of deviated genotypes linked with selection pressures and selection methods.These functional markers and genotypes related to the traits may be used for marker assisted selection and provided the efficient evidence of genetic mechanism on growth traits in common carp.

The sequence homology and molecular evolution of Hoxa3a gene were analyzed in common carp and other fishes and organisms by some bioinformatics softs.The results showed that the maximal sequence homology（up to 95%） was observed in common carp（Cyprinus carpio） and zebrafish（Danio rerio）,followed by Atlantic salmon（Salmo salar）,Japanese pufferfish（Takifugu rubripes）,Japanese medaka（Oryzias latipes） and Cichlid fish（Astatotilapia burtoni）（about 84%）,and the minimal（74.5%） in other fishes such as lttle skate（Leucoraja erinacea） and Australian ghost shark（Callorhinchus milii）.The sequence homology of common carp with three non-fish organisms was found to be 75%.This result also showed that Hox gene sequences were highly conserved evolutionarily.In the sequence comparison at the same time,two common carp SNP sites 101 site（T/C） and 213 site（C/T） were found.The result has great significance for the conservation of biological diversity（particularly genetic diversity） study and reveals the progress and mechanism of biological evolution.

The PCR technique was used to amplify the mtDNA D-Loop in 30 individuals of Paramisgurnus dabryanus collected from the Nansi Lake.The PCR products were purified and sequenced,and then 963bp nucleotide sequences of partial D-Loop were obtained.CLUSTAL X（1.83） to align and comparison of the sequences of these 30 individuals with each other revealed that there were 33 variation sites including 12 transition sites and 21 transversion sites.The pairwise genetic distances were computed by MEGA,and the UPGMA and NJ phylogenetic trees was established through the cluster analysis over the pairwise genetic distance.DNASP showed that the number of haplotypes（h） was found to be 15,the haplotype diversity（H） 0.899,nucleotide diversity（Pi） 0.008 and the average number of nucleotide diferences（K） 7.79.It can be concluded that the variation in mtDNA D-Loop of Paramisgurnus dabryanus in the Nansi Lake is relatively rich and wealthy on genetic diversity.

In this study,four isozymes（EST,AMY,LDH and MDH） in three kinds of tissues（liver,kidney and eye） of Barbus Capito were analyzed using the vertical plate polyacrylamide gel electrophoresis（PAGE）.The results showed that the expression of isozymes exhibited apparent tissue-specific.Enzyme activity was the highest in the liver,where has all forms of that enzyme band.

As a spare water head site of domestic water resources for Harbin,The water quality of Xiquanyan Reservoir is closely related to water security of millions of citizens.The upstream of Ashi River basin is the main sub basin of Xiquanyan reservoir and its water quality directly affect water quality of the reservoir.The water quality in upper reaches of Ashi River was assessed by the methods of single factor pollution index and grey connecting evaluation method based on the monitored physical and chemical index of the water quality in September 2010.The results showed that the water quality in all sampling points was in GradeⅠexcept for R8（Grade Ⅱ）.Grey evaluation method was found superior to single-factor method for the evaluation,which was closer to the actual situation of upper Ash River basin.Single-factor pollution index only revealed pollution degree of certain pollutant instead of the whole water body.Meanwhile,the Gray evaluation method will take advantage of environmental monitoring data and comprehensively analyzed the water pollution better.

The developmental history of sturgeon aquaculture industry and cultural technological research in China were described by research practices of the authors and the relevant data,and developing tendency was introduced,particularly focusing on the aquaculture species,aquaculture areas,production manners,production and industry development trend.The proposals on the primary challenges of the industry development were discussed in the article.